Human VAP-1 Quantikine ELISA Kit
Human VAP-1 Quantikine ELISA Kit
貨號：DVAP10 深圳子科生物優(yōu)勢代理美國R&DELISA系列產品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
Human VAP-1 Quantikine ELISA Kit Summary
貨號：DVAP10 規(guī)格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human VAP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human VAP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VAP-1 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human VAP-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VAP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VAP-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
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Inter-Assay Precision
|
|
Sample
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1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
3.41
|
10.6
|
22.7
|
3.96
|
11.8
|
24.6
|
|
Standard Deviation
|
0.071
|
0.163
|
0.535
|
0.18
|
0.567
|
1.16
|
|
CV%
|
2.1
|
1.5
|
2.4
|
4.5
|
4.8
|
4.7
|
Recovery
The recovery of VAP-1 spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
|
|
Cell Culture Media (n=4)
|
104
|
95-114
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of VAP-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
