Canine VEGF Quantikine ELISA Kit
Canine VEGF Quantikine ELISA Kit
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Canine VEGF Quantikine ELISA Kit Summary
貨號：CAVE00 規(guī)格:96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Assay Range
19.5 - 1,250 pg/mL (Cell Culture Supernates), 39.1 - 2,500 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant canine VEGF. This assay cross-reacts with Human VEGF165.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Canine VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant canine VEGF and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine VEGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine VEGF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
29.1
|
123
|
531
|
32.8
|
128
|
495
|
|
Standard Deviation
|
1.9
|
5
|
18.4
|
2.8
|
6.4
|
33
|
|
CV%
|
6.5
|
4.1
|
3.5
|
8.5
|
5
|
6.7
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
53.7
|
235
|
910
|
64.5
|
250
|
1003
|
|
Standard Deviation
|
3.6
|
10.6
|
46.2
|
5.7
|
17.4
|
61.7
|
|
CV%
|
6.7
|
4.5
|
5.1
|
8.8
|
7
|
6.2
|
Recovery
The recovery of canine VEGF spiked to three different levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
102
|
94-110
|
|
EDTA Plasma (n=4)
|
98
|
92-107
|
|
Heparin Plasma (n=4)
|
107
|
96-118
|
|
Serum (n=4)
|
103
|
87-113
|
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine VEGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
