Human u-Plasminogen Activator/Urokinase Quantikine ELISA
Human u-Plasminogen Activator/Urokinase Quantikine ELISA
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Human u-Plasminogen Activator/Urokinase Quantikine ELISA Summary
貨號：DUPA00 規(guī)格:96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL), Urine (10 uL)
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human uPA. This assay also recognizes uPA complexed with PAI-1 or uPAR.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human u-Plasminogen Activator/Urokinase Immunoassay is a 4.5 hour solid phase ELISA designed to measure uPA in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human uPA and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human uPA. Results obtained using natural human uPA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human uPA.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
230
|
629
|
1219
|
214
|
614
|
1188
|
|
Standard Deviation
|
4.8
|
8.9
|
29.3
|
15.1
|
39.8
|
82
|
|
CV%
|
2.1
|
1.4
|
2.4
|
7.1
|
6.5
|
6.9
|
Recovery
The recovery of uPA spiked to three different levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=8)
|
102
|
93-115
|
|
Cell Lysates (n=4)
|
97
|
81-116
|
|
EDTA Plasma (n=4)
|
87
|
83-94
|
|
Heparin Plasma (n=4)
|
89
|
81-110
|
|
Serum (n=4)
|
87
|
81-93
|
|
Urine (n=4)
|
98
|
81-119
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of uPA were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
