Human Cystatin C Quantikine ELISA Kit
Human Cystatin C Quantikine ELISA Kit
Human Cystatin C Quantikine ELISA Kit Summary
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貨號(hào)：DSCTC0
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL), Human Milk (50 uL)
Assay Range
3.1 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human Cystatin C
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Cystatin C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cystatin C in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Cystatin C and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cystatin C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Cystatin C.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
16.2
|
29.9
|
52.6
|
17.2
|
30.8
|
60.9
|
|
Standard Deviation
|
1.07
|
0.92
|
2.41
|
1.21
|
1.53
|
3.6
|
|
CV%
|
6.6
|
3.1
|
4.6
|
7
|
5
|
5.9
|
Recovery
The recovery of Cystatin C spiked to levels throughout the range of the assay was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
Cell Culture Media (n=4)
|
96
|
88-102
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of Cystatin C were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
