Glucagon Quantikine ELISA Kit
Glucagon Quantikine ELISA Kit
Glucagon Quantikine ELISA Kit Summary
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貨號：DGCG0
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural Glucagon. This assay cross-reacts <12% with Oxyntomodulin.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Glucagon Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Glucagon in cell culture supernates, serum, and plasma. It contains natural porcine Glucagon as the standard. The antibodies were raised against a human Glucagon synthetic peptide. This immunoassay has been shown to accurately quantitate human, mouse, rat, and porcine Glucagon.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
315
|
618
|
1024
|
354
|
653
|
1080
|
|
Standard Deviation
|
10.3
|
22
|
27.6
|
30.7
|
37.8
|
63.6
|
|
CV%
|
3.3
|
3.6
|
2.7
|
8.7
|
5.8
|
5.9
|
Recovery
The recovery of Glucagon spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
98
|
91-108
|
|
Human EDTA Plasma (n=4)
|
98
|
86-107
|
|
Human Heparin Plasma (n=4)
|
96
|
88-100
|
|
Human Serum (n=4)
|
102
|
95-111
|
|
Mouse Serum (n=2)
|
99
|
92-106
|
|
Porcine Serum (n=2)
|
97
|
91-107
|
|
Rat Serum (n=1)
|
93
|
86-101
|
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Glucagon were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
