Human TNF RI/TNFRSF1A Quantikine ELISA Kit
Human TNF RI/TNFRSF1A Quantikine ELISA Kit
Human TNF RI/TNFRSF1A Quantikine ELISA Kit Summary
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貨號：DRT100
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Citrate Plasma (20 uL), Urine (20 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human sTNF RI
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human sTNF RI Immunoassay is a 4.5 hour solid phase ELISA designed to measure sTNF RI in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human sTNF RI, as well as antibodies raised against this polypeptide. The recombinant protein represents the non-glycosylated, N-terminal methionyl form of the naturally occurring human soluble Type I receptor for TNF with an apparent molecular weight of approximately 18.6 kDa. This immunoassay has been shown to accurately quantitate the recombinant sTNF RI. Results obtained on samples containing natural sTNF RI showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural sTNF RI. Since the measurement of human sTNF RI by this immunoassay is relatively insensitive to added TNF-alpha or TNF-beta, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples (i.e. the total amount of free receptor plus the total amount of receptor bound to TNF).
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
41.5
|
122
|
231
|
54
|
251
|
350
|
|
Standard Deviation
|
2.14
|
5.38
|
11
|
2.7
|
9.7
|
23.3
|
|
CV%
|
5.2
|
4.4
|
4.8
|
5
|
3.9
|
6.7
|
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
69
|
198
|
355
|
54.8
|
252
|
356
|
|
Standard Deviation
|
3.24
|
7.17
|
17.8
|
4.8
|
9.3
|
20.6
|
|
CV%
|
4.7
|
3.6
|
5
|
8.8
|
3.7
|
5.8
|
Recovery
The recovery of sTNF RI spiked to three levels in samples throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=8)
|
85
|
80-92
|
|
EDTA Plasma (n=8)
|
86
|
70-97
|
|
Heparin Plasma (n=8)
|
93
|
79-103
|
|
Serum (n=8)
|
90
|
77-103
|
|
Urine (n=8)
|
85
|
71-109
|
