Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit Summary
Catalog Size / Price
MJE00 1 Kit (2 Plates) / Please Inquire
SMJE00 1 Pack (6 Plates) / Please Inquire
PMJE00 1 Pack (50 Plates) / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (25 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse and rat MCP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat CCL2/MCP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MCP-1 in mouse or rat cell culture supernates and serum. It contains E. coli-expressed mouse MCP-1 and antibodies raised against the recombinant factor. This Immunoassay has been shown to accurately quantitate recombinant mouse MCP-1. Results obtained using natural mouse or rat MCP-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural MCP-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
47.1
|
311
|
768
|
43.6
|
310
|
783
|
|
Standard Deviation
|
3.9
|
15.8
|
40.6
|
3.2
|
14.3
|
42.8
|
|
CV%
|
8.3
|
5.1
|
5.3
|
7.3
|
4.6
|
5.5
|
Recovery
The recovery of MCP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Mouse Cell Culture Supernates (n=6)
|
104
|
91-119
|
|
Mouse Serum (n=9)
|
99
|
89-113
|
|
Rat Cell Culture Supernates (n=4)
|
109
|
103-115
|
|
Rat Serum (n=4)
|
109
|
103-116
|
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